Blood cells
Stained with diff-quick
|
Technique |
|||||||
|
|
Make a smear of a drop of blood and air dry |
||||||
|
Fix in Diff-Quick solution A for 10 minutes |
|||||||
|
Dip slides 25 times in Solution B – allow slide to remain in solution B for total of 10 minutes. Do not rinse slide |
|||||||
|
Dip slide 25 times in solution C for a total of 25 minutes |
|||||||
|
Wash with Phosphate buffered saline or distilled, deioized water |
|||||||
|
|
Permanent fixation: |
||||||
|
Air dry slides. Clear in Xylene and mount using synthetic mounting medium |
|||||||
|
Blood cells differentiation |
|||||||
|
|
2 1 |
|
|||||
|
General blood smear |
Erythrocycte (arrow 1) and platelet (arrow 2) |
|
|||||
|
|
|
|
|||||
|
Neutrophil- note the segmented nucleus |
Monocycte – Large nucleus filling cell |
Eosinophil- note the red granules Basophils – look similar but with red granules |
|||||
White blood cells are made up of the combination of Lymphocyctes plus the neutrophils, monocyctes, eosinophils and basophils